Identification Of Unknown Bacteria Using Biochemical Test

When bacteria is grown in this medium there may be the production of acids or alkali or even no change in ph.

There are many biochemical tests available for bacterial identification. Differential media like blood agar is useful in identifying the type of haemolysis and thus the pathogenicity of various bacteria streptococci. The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture. In the first step in the biochemical identification students use a single colony to streak an emb lactose agar plate to determine if their unknown is gram.

Students then use these plates to make their own unknown plate by streaking for single colonies. Few of them are required to be carried out depending upon the bacteria. This test is used to determine the presence of bacterial cytochrome oxidase. Identification of gram positive bacteria can be achieved by carrying out various biochemical tests.

This is based on the following chain of logic. Automated methods of identification of unknown bacteria. It produces benefits for many aspects of the research of microorganisms and helps physicians correctly treat patients. This test is done to help identify proteus morganella yersinia enterocolitica and helicobacter pylori.

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Bacteria is grown in a sugar media. This is one of the widely used methods and more important. Identification of bacteria by biochemical tests. To identify bacteria we must rely heavily on biochemical testing.

A different unknown number is given to each place bacteria are collected. Each different species of bacterium has a different molecule of dna i e dna with a unique series of nucleotide bases. Identification of unknown bacteria by the manual method using staining reactions motility test cultural characteristics and biochemical tests is a cumbersome lengthy and time consuming process. Urease test is used to determine the ability of an organism to produce urease an enzyme which hydrolyzes urea.

The types of biochemical reactions each organism undergoes act as a thumbprint for its identification. Iii biochemical tests the staining is followed by use of various biochemical reagents and tests to get closer to the identification of bacteria.